- Title
- Oxidized vitamin E and glutathione as markers of clinical status in asthma
- Creator
- Wood, Lisa G.; Garg, Manohar L.; Blake, Robert J.; Simpson, Jodie L.; Gibson, Peter G.
- Relation
- Clinical Nutrition Vol. 27, Issue 4, p. 579-586
- Publisher Link
- http://dx.doi.org/10.1016/j.clnu.2007.12.002
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2008
- Description
- Background & aims: Antioxidant status is disturbed in asthma. Measurement of both oxidized and reduced forms of antioxidants provides important information regarding the oxidant/antioxidant balance. The aim of this study was to investigate the clinical relevance of key antioxidants (α-tocopherol and glutathione) in asthma, by measuring the oxidized and reduced forms, in the airways (induced sputum) and systemically (peripheral blood). Methods: This cross-sectional study examines stable asthmatics (n=44) and healthy controls (n=31) recruited through John Hunter Hospital, NSW, Australia. We collected peripheral blood and induced sputum during hypertonic saline challenge. α-tocopherol and α-tocopherol quinone were measured by HPLC. Total glutathione and glutathione disulfide were determined by a colorimetric assay. Results: Plasma α-tocopherol was low in asthma versus controls. Subjects with asthma had higher levels of whole blood α-tocopherol quinone and %α-tocopherol quinone than controls and %α-tocopherol quinone correlated with asthma control (p=0.009). Sputum supernatant levels of total, reduced and oxidized glutathione were elevated in asthma versus controls. Oxidized glutathione in sputum supernatant negatively correlated with FEV₁/FVC% (p=0.029).
- Subject
- α-tocopherol; α-tocopherol quinone; antioxidants; asthma; glutathione; glutathione disulfide
- Identifier
- http://hdl.handle.net/1959.13/41527
- Identifier
- uon:4850
- Identifier
- ISSN:0261-5614
- Language
- eng
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